Journal: bioRxiv

Article Title: Structural basis of rotavirus RNA chaperone displacement and RNA annealing

doi: 10.1101/2020.10.26.354233

Figure Lengend Snippet: A. RT-PCR results of the RNA extracted from MA104 cells infected with lysates from reverse genetics experiments designed to rescue C-terminally 6xHis-tagged NSP2 (panel B), and NSP2-EED and NSP2-AAA mutants (panel C). For each experiment, three independent attempts were made to rescue the wild-type virus and the mutants. Total RNA was extracted from virus-infected cells for each mutant (Materials and Methods), and amplified using gs8-specific primers, prior to further verification by sequencing. For gs8-NSP2-AAA mutant, sequencing data are shown only for the plasmid gs8-NSP2-AAA used for the virus rescue, as no cDNA could be made due to the absence of replicating viral RNA, even after two blind passages (panel A, AAA mutant, lane P3).

Article Snippet: MA104 (embryonic African green monkey kidney cells, ATCC® CRL-2378) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) (Life Technologies) supplemented with 10% Fetal Bovine Serum (FBS) (Life Technologies).

Techniques: Reverse Transcription Polymerase Chain Reaction, Infection, Virus, Mutagenesis, Amplification, Sequencing, Plasmid Preparation

Journal: iScience

Article Title: Comparison of DNA vaccines with AS03 as an adjuvant and an mRNA vaccine against SARS-CoV-2

doi: 10.1016/j.isci.2023.107120

Figure Lengend Snippet: The recombinant Spike Protein as a DNA-vaccine candidate for SARS-CoV-2 (A) Schematic representation of the prefusion-stabilized SARS-CoV-2 HexaPro ectodomain showing the S1 and S2 subunits. Four additional proline substitutions from the S-2P construct are indicated by the red arrows shown below the construct. SS- Signal sequence; NTD N-terminal domain; RBD- Receptor Binding domain; SD1-2- Subdomain1-2; CH- Central helix; CD-connector domain; HR-heptad repeat FP- fusion peptide. (B) HexaPro Spike protein expressed in Expi293 cells was confirmed by SDS-PAGE. (C–D) His-tagged HexaPro was expressed in Expi293, purified and characterized by SDS-PAGE (right). (E) Expression of HexaPro Spike confirmed by western blot using a commercial anti-His antibody or pooled sera of Spike-immunized mice. (F) Flow cytometric analysis showing the binding of pooled mouse sera of HexaPro immunized mice to the HexaPro Spike expressed on 293T cells. (G–H) The mRNA expression levels of inflammatory cytokines IL-6 and TNFα in HexaPro-transfected cells were detected by qRT-PCR. The bars represent the means with error bars denoting the SD of three samples (∗∗∗significantly different (p < 0.001) by two-tailed unpaired t-test).

Article Snippet: BEAS2B, 293T cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Recombinant, Construct, Sequencing, Binding Assay, SDS Page, Purification, Expressing, Western Blot, Transfection, Quantitative RT-PCR, Two Tailed Test

Journal: iScience

Article Title: Comparison of DNA vaccines with AS03 as an adjuvant and an mRNA vaccine against SARS-CoV-2

doi: 10.1016/j.isci.2023.107120

Figure Lengend Snippet:

Article Snippet: BEAS2B, 293T cells were obtained from American Type Culture Collection (Manassas, VA).

Techniques: Virus, Recombinant, Adjuvant, Cell Stimulation, Modification, Expressing, Neutralization, Double-Color Enzymatic ELISPOT, cDNA Synthesis, Transfection, Purification, Plasmid Preparation, Enzyme-linked Immunosorbent Assay, Software

Journal:

Article Title: The Long Terminal Repeat of Jaagsiekte Sheep Retrovirus Is Preferentially Active in Differentiated Epithelial Cells of the Lungs

doi:

Figure Lengend Snippet: Cell lines used in this study

Article Snippet: H441 and H358 cells were grown in RPMI 1640 medium (Gibco BRL) adjusted to contain 1.5 g of sodium bicarbonate per liter, 4.5 g of glucose per liter, and 10 mM HEPES with 10% FBS. table ft1 table-wrap mode="anchored" t5 TABLE 1 caption a7 Cell line Origin Tissue or cell type ATCC no. or reference A549 Human Lung carcinoma CCL-185 H441 Human BAC HTB-174 H358 Human BAC CRL-5807 293T Human Embryonic kidney Lebkowsky et al. ( 33 ) OA1 Sheep Brain fibroblast CRL-6538 OAT Sheep Sheep testis CRL-6546 FLL Sheep Primary fetal lamb MRI a JS7 Sheep BAC Jassim ( 30 ) CP-MRI Sheep Choroid plexus MRI a CP-ATCC Sheep Choroid plexus CRL-1700 mtCC1-2 Mouse Clara cell Magdaleno et al. ( 34 ) BV2 Mouse Microglia A. Tenner a F9 Mouse Testicular carcinoma CRL-1720 FOP Mouse Mammary carcinoma J. Hassel a IC-21 Mouse Peritoneal macrophage TIB-186 MHS Mouse Alveolar macrophage CRL-2019 C2C12 Mouse Myoblast CRL-1772 ABI-2 Mouse Hybridoma HB-33 MLE-12 Mouse Lung epithelium CRL-2110 TCMK Mouse Mouse kidney CCL-139 NIH 3T3 Mouse Mouse embryo CCL-92 MLE-15 Mouse Type II pneumocyte Wikenheiser et al. ( 59 ) ST3 Mouse Thymus stroma Brightman et al. ( 10 ) Open in a separate window a Cells were provided directly by an investigator or institution with no reference available.

Techniques:

Journal: Cell

Article Title: TMEM41B Is a Pan-flavivirus Host Factor

doi: 10.1016/j.cell.2020.12.005

Figure Lengend Snippet:

Article Snippet: Human: HEK293T/17 (embryonic kidney epithelial) , ATCC , Cat.#CRL-11268 ; RRID: CVCL_1926.

Techniques: Virus, Subcloning, Western Blot, Recombinant, Infection, Transfection, Protease Inhibitor, Staining, Bicinchoninic Acid Protein Assay, Immunoprecipitation, SYBR Green Assay, cDNA Synthesis, Sequencing, Derivative Assay, Plasmid Preparation, Software